Cloning of human bone morphogenetic protein-2(hBMP-2) cDNA by PCR method.
نویسندگان
چکیده
منابع مشابه
Accelerators of Osteogenesis by Recombinant Human Bone Morphogenetic Protein-2
Bone morphogenetic protein (BMP) appears to be one of the most promising cytokine and for clinical use in reconstructive surgery for bony defects and augmentation. To evaluate the effect of basic fibroblast growth factor (bFGF), FK506, elcatonin, and hyperbaric oxygenation (HBO) on osteoinduction by recombinant human bone morphogenetic protein-2 (rhBMP-2), 2 or 5 μg of rhBMP-2 was implanted int...
متن کاملmolecular cloning of bone morphogenetic protein-2 (bmp- 2) in pgem-b1 vector and transformation into e.coli rosetta strain
background: bone morphogenetic protein 2 (bmp- 2) belongs to the tgf-β superfamily of proteins and plays an important role in the development of bone and cartilage. bmp- 2 is also associated with maintenance and repair of damaged bone. recombinant human bone morphogenetic protein 2 (rhbmp- 2) is now produced by genetic engineering techniques and used in treatment of thin bone fractures in the j...
متن کاملEnhanced bone augmentation by controlled release of recombinant human bone morphogenetic protein-2 from bioabsorbable membranes.
BACKGROUND The present study was undertaken to determine the effect of recombinant human bone morphogenetic protein-2 (rhBMP-2)-loaded biodegradable membranes on bone augmentation in a rabbit calvarial model. METHODS Five microg of rhBMP-2 was loaded into a stiff hemispherical dome membrane made of poly(L-lactide) and tricalcium phosphate (PLLA/TCP). The release kinetics of rhBMP-2 from the m...
متن کاملRegulation of scleral fibroblast differentiation by bone morphogenetic protein-2.
Bone morphogenesis proteins (BMPs) are multi-functional growth factors. They are expressed in retina, retinal pigment epithelium (RPE) and sclera and serve as a regulator in the growth and development of the eye. This article reviewed the chondrogenic potency of the sclera, biochemical and pathological changes of myopic scleral tissue and the differentiation of chondrogenesis by BMP-2. We propo...
متن کاملBone morphogenetic protein-2 stimulates Runx2 acetylation.
Runx2/Cbfa1/Pebp2aA is a global regulator of osteogenesis and is crucial for regulating the expression of bone-specific genes. Runx2 is a major target of the bone morphogenetic protein (BMP) pathway. Genetic analysis has revealed that Runx2 is degraded through a Smurf-mediated ubiquitination pathway, and its activity is inhibited by HDAC4. Here, we demonstrate the molecular link between Smurf, ...
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ژورنال
عنوان ژورنال: Japanese Journal of Oral Biology
سال: 1993
ISSN: 0385-0137
DOI: 10.2330/joralbiosci1965.35.64